DeLong:lLab Protocols:Julie's protocols

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Julie's Protocols

A warning: these all work for me, but molecular biology is a mysterious thing, so they may not work for you! Please email me at jmaresca (at) mit (dot) edu if you have a question or a problem. Thanks.

Preparation of electrocompetent cells. This works for E. coli, and I have not tested it on any other bacterial species. File:Competent cells.pdf

Plasmid Minipreps. This is a standard miniprep protocol. File:PlasmidMini.pdf

Standard PCR reactions (for normal amplification and for sequencing). File:PCR jm.pdf

Use of the sonicator for small bacterial samples. File:Delong Sonicator.pdf

Reverse-phase HPLC analysis of carotenoids and (bacterio)chlorophylls. File:HPLC Tokyo.pdf

Reverse-phase HPLC analysis of carotenoids produced in E. coli. File:HPLC Ecoli.pdf