DIYbio:Notebook/Open Thermal Cycler
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Why quantitative thermal cycling?
How do you go from a spit sample to - or from a crosswalk swab to a map of the bacterial populations of the world? Can $1,000 get you there? quantitative thermal cycling can be an all-in-one device capable
Comparisons to standard thermal cycling
The following is the outline of quantitative thermal cycling, its advantages over traditional thermal cycling,
There are 2 general areas of thermal cycler use:
Since thermal cycling itself is not an analytical tool, thermal cycling is not the final step in most processes. Gel electrophoresis, transformation into cells, and/or sequencing of the sample follow.
There are 2 general areas of quantitative thermal cycler use:
qPCR on the other hand can be an all-in-one tool for analysis, both qualitatively and quantitatively. In addition to the functionality of a standard thermal cycler, a quantitative thermal cycler includes an imaging device to record the florescence of samples. Florescent markers added to each sample indicate the presence of a particular sequence (replacing gel electrophoresis and short sequencing) or measure gene expression (supplementing other analysis of modified cells)
Cost of current products
Use Cases - experiments involving a thermal cycler
Requirements- what a user might want to do with an Open Thermal Cycler (i.e. get more DNA)
Specifications - what hardware, software, GUI-ideas we might use to build it (i.e. Arduino, or a checkbox interface for entering temperatures)
4/26/2009 Conference Call - Details for connecting to the conference call.
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