Cong T. Trinh:media

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Acetobactor aceti

YPD (Uwe sauer)

Chemicals      Concentration
glucose	        30 g/L	
bacto peptone	2 g/L	
yeast extract	5 g/L	
  • Grow at 30°C

ATCC medium

Chemicals      Concentration
yeast extract	5 g/L
peptone	        3 g/L
mannitol	25 g/L



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E. coli

2 LB++

Chemicals      Concentration
2xLB     	-see below
MgSO4	        2 g/L
NH4SO4	        1 g/L
Glucose	20 g/L

910 Broth

Chemicals      Concentration
(NH4)2HPO4	10 g/L
K2HPO4	        5 g/L
Na2SO4	        0.5 g/L
Octane	        1% (v/v)

B00BIES

Chemicals      Concentration
Tryptone	10 g/L
Yeast Extract   5 g/L
5x M9 Salts     200 mL/L
1M MgSO4        2 mL/L
1M CaCl2        0.1 mL/L

LB(Low Salt/ general use)

Chemicals      Concentration
Tryptone	10 g/L
Yeast Extract  5 g/L
NaCl	        5 g/L

LB(plates)

Chemicals      Concentration
Tryptone	10 g/L
Yeast Extract   5 g/L
NaCl	        5 g/L
Agar            20 g/L

M9 Minimal Media

Chemicals      Concentration
Make M9 salts
  To make M9 Salts aliquot 800ml H2O and add
  64g Na2HPO4-7H2O
  15g KH2PO4
  2.5g NaCl
  5.0g NH4Cl
  Stir until dissolved
  Adjust to 1000ml with distilled H2O
Sterilize by autoclaving
Measure ~700ml of distilled H2O (sterile)
  Add 200ml of M9 salts
  Add 2ml of 1M MgSO4 (sterile)
  Add 20 ml of 20% glucose (or other carbon source)
  Add 100ul of 1M CaCl2 (sterile)
  Adjust to 1000ml with distilled H2O

Minimal buffer A for random mutagenesis

Chemicals      Concentration
K2HPO4	        10.5 g/L
KH2PO4	        4.5 g/L
(NH4)2SO4	1.0 g/L
NaCIT.2H2O	0.5 g/L


SOB Media

Chemicals      Concentration
Tryptone	20 g/L
Yeast extract	5 g/L 
NaCl	        0.5 g/L
KCl	        0.2 g/L
MgSO4	        2.4 g/L
pH	        7

SOC MEdia

Same as SOB + 20mM Glucose


Stock trace metals solution

Chemicals      Concentration
H3BO4	        0.15 g/L
CoSO4   	0.065 g/L
ZnSO4.7H2O	0.05 g/L
MnCl2.4H2O	0.015 g/L
NaMo4.2H2O	0.015 g/L
NiCl2.6H2O	0.01 g/L
CuSO4.5H2O	0.005 g/L
Fe(NH4)Citrate	3 g/L


Z broth

Chemicals      Concentration
LB             50mL 
CaCl2          25μL of 1M CaCl2

Antibiotic Working Concentration

Antibiotic         Concentration 
Ampicillin          100 μg/mL              
Kanamycin           50 μg/mL
Chloramphenicol     25 μg/mL
Tetracycline        50 μg/mL
Amp+Kan             25 μg/mL of each




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P. putida

Normal growth media

Chemicals      Concentration
LB 
glucose        20 g/L
  • Grow at 30°C



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Z. mobilis

ATCC medium

Chemicals      Concentration
yeast extract	10 g/L
glucose	20 g/L
KH2PO4	        2 g/L
  • adjust pH to 6.5

PL Rogers

Chemicals      Concentration
yeast extract	10 g/L
glucose	        20 g/L
MgSO4.7H2O	1 g/L
KH2PO4	        2 g/L
(NH4)2SO4	1 g/L



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