Chang Lab:Notebook/CBE/08/149/2008/12/23

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Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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  • As per procedure, reading of CV absorbance of Trial IV biofilms

Biofilm Optimization Trial V

  • Same procedure as Trial III, with minor addendum. CONDITIONS: 21 C and 37 C for 72 hrs
  • Addendum: grew 5-hr inoculum using M9 medium, to check (via CV readings) whether there is an effect on biofilm growth.

Sonication step

  • Ultrasonic bath in the lab too strong for biofilm removal.
  • Utilized sonicator probe in Prof Kunn's Lab ==> too weak.
  • Purchase lids with detachable pegs.

Plate count on 1.0 McFarland LB and M9 of Trial IV (12/20)

  • LB:
    • 10^-12: 105
    • 10^-14: 50
  • M9:
    • 10^-16: 359