Chang Lab:Notebook/CBE/08/149/2008/12/17

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Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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  • Start: 8:30 AM
  • Cultured bacteria (for biofilm optimization trial III next day)
    • Single loop taken from streaked petri dishes on 12/13 and suspended into 10ml broth in a test tube (incubate at 37 C for 24hrs).

Notes on serial plating

  • Plates incubated on 12/15: Sonicated biofilms from Trial I
    • No growth at 10^-6, 10^-8, 10^-10 dilutions ==> REDUCE dilutions for Trial II plating
  • Plates incubated on 12/16: Biofilm Inoculum (matched at 1.0 McFarland)
    • LB: Too many to count at 10^-10, 10^-12, 10^-14 ==> INCREASE dilutions for Trial III plating
    • M9: No growth at 10^-10, 10^-12, 10^-14 ==> Need to rethink centrifuge procedure!!!