- The incubated PA01 strain from 19/01/09 did not turn turbid meaning that was no growth.
- Prepared 4 new test tubes of PA01 strains from different PA01 cryogenic stock to check their variability.
- The 4 choosen were PA01 2-5.
- Same procedure was repeated as 19/01/09 where the cryogenic stock was thawed.
- Next 0.15ml of the PA01 strain was added to 3ml of LB broth in a test tube and incubated at 37 C. 250rpm overnight.