Chang Lab:Notebook/CBE/08/146/2009/01/19

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Time started:4pm

P.aeruginosa inoculum preparation:

  • Thawed the cryogenic sample from the freezer till liquid.
  • Added 0.1ml of the PA01 strain to 9ml of LB broth.
  • Incubated for 24 hrs (instead of the usual 6 hrs as the strain was kept for over a year already) at 37 C, 250rpm.