- Cultured bacteria (for biofilm optimization trial III next day)
- Single loop taken from streaked petri dishes on 13/12/08 and suspended into 10ml broth in a test tube (incubate at 37 C for 24hrs).
- Plates incubated on 15/12: Sonicated biofilms from Trial I
- No growth observed for 10^-6, 10^-8, 10^-10 dilutions; need to reduce dilutions for Trial II plating.
- Plates incubated on 16/12/08: Biofilm Inoculum (matched at 1.0 McFarland)
- LB: Too many to count at 10^-10, 10^-12, 10^-14; need to increase dilutions for Trial II plating
- M9: No growth at 10^-10, 10^-12, 10^-14 (questionable fault with the centrifuge procedure).