Chang Lab:Notebook/CBE/08/146/2008/12/17

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Entry title

Time start:830am

  • Cultured bacteria (for biofilm optimization trial III next day)
    • Single loop taken from streaked petri dishes on 13/12/08 and suspended into 10ml broth in a test tube (incubate at 37 C for 24hrs).

Note

  • Plates incubated on 15/12: Sonicated biofilms from Trial I
    • No growth observed for 10^-6, 10^-8, 10^-10 dilutions; need to reduce dilutions for Trial II plating.
  • Plates incubated on 16/12/08: Biofilm Inoculum (matched at 1.0 McFarland)
    • LB: Too many to count at 10^-10, 10^-12, 10^-14; need to increase dilutions for Trial II plating
    • M9: No growth at 10^-10, 10^-12, 10^-14 (questionable fault with the centrifuge procedure).