Biomod/2012/HKBU/BU Magician:Labook:Notebook/Labbook/2012/10/01

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2012/10/01

MTT Treatment

MTT Solution Adding and Data Reading
1. Medium was discarded and washed by PBS two times.
2. 200ul MTT-medium solution was added in a concentration of 250ug/ml in dark at 37°C for 3 hours.
3. MTT-medium solution was discarded and replaced by DMSO.
4. The 96 well plate was shake for 15 minutes until solid crystal attached in each well was dissolved.
5. Absorbanced was measured at 540nm and 690nm using BioTek.