Biomod/2011/TUM/TNT/LabbookA/2011/09/21

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21th Sep 2011 <!-- sibboleth --><div id="m1" style="border:0px;"><div style="display:none;" id="id">m1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">Biomod/2011/TUM/TNT/Labbook/20</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month">8</div><div style="display:none;" id="year">2011</div><div style="display:none;" id="readonly">Y</div></div> <!-- sibboleth --><div id="m2" style="border:0px;"><div style="display:none;" id="id">m2</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">Biomod/2011/TUM/TNT/Labbook/20</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month">9</div><div style="display:none;" id="year">2011</div><div style="display:none;" id="readonly">Y</div></div> <!-- sibboleth --><div id="m3" style="border:0px;"><div style="display:none;" id="id">m3</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">Biomod/2011/TUM/TNT/Labbook/20</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month">10</div><div style="display:none;" id="year">2011</div><div style="display:none;" id="readonly">Y</div></div>

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Observed TEM Grids from 2011/09/16

BM2 with 25µM EtBr is strongly deformed in comparison to BM2 without DNA binders (see Fig. 1):
File:110921 theU BM2 004 16.tif File:110921 theU BM2 25uM EtBr013 16.TIF
Fig.1:
BM2 without DNA binders (left); BM2 with 25µM EtBr (right)


With 75µM, no origamis can be found, but only structures in the background, probably fragments of destroyed origamis due to the high concentration of EtBr (Fig. 2 left). 100µM spermine cause massive aggregation of the structures, as expected from the previous measurements at the fluorescence microscope (Fig. 2 right).

File:110921 theU BM2 75uM EtBr005 16.TIF File:110921 theU BM2 100uM Spermin005 16.TIF
Fig.2:

BM2 with 100µM EtBr (left); BM2 with 100µM spermine (right)