BME100 f2014:Group30 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable is the amount of lipopolysaccharide given, since the lipopolysaccharide is the variable that will likely change or affect the dependent variable. The dependent variable is the amount of inflammatory protein, since the protein in each test subject is the variable that is influenced by the lipopolysaccharide, the independent variable.
We will randomly select subjects from groups of male and female elders that both consist of a range of ages, and histories of inflammatory issues. We will give each of the possible subjects numbers and put the numbers in a hat to randomly select the 10 subjects for each testing groups. The selection from male and female groups will help to avoid any bias in terms of gender, and the selection of numbers from the hat will help to further randomize the data collection for the experiment.
Sources of Error and Bias
Possible sources of bias or error include: 1) Varying routines - Different doses of lipopolysaccharide could effect subjects differently due to different routines during the day, and different amounts of activity 2) Genetic differences - Subjects could react to the different doses of lipopolysaccharide due to differences in genetic make up 3) Gender differences - Males could react to the different doses of lipopolysaccharide differently than females do.
The only way to avoid these possible sources of error is to randomize the selection of subjects so that each testing group is diverse, and implement the correct control groups. There will be a control group that is also randomly selected for, which will represent all of the possible sources of error listed above in a controlled fashion.