BME100 f2017:group16 W1030 l4

BME 100 Fall 2017	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 4 WRITE-UP [BME 100: PCR LAB] Protocol LAB A: SECTION 1- Materials PPE:Lab coat (proper attire),gloves,glasses PCR mixture: 8 tubes, 50 μL each of Taq DNA polymerase, MgCl2, and dNTP’s DNA/ primer mix: 8 tubes, 50 μL each different templates of DNA Empty PCR tubes Disposable pipettes Cup (for scrap tips) Micropipettor OpenPCR machine PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G# + Positive control none G# - Negative control none G# 1-1 Patient 1, replicate 1 G# 1-2 Patient 1, replicate 2 G# 1-3 Patient 1, replicate 3 G# 2-1 Patient 2, replicate 1 G# 2-2 Patient 2, replicate 2 G# 2-3 Patient 2, replicate 3 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program Research and Development PCR - The Underlying Technology SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing