BME100 f2016: Group2 W1030AM L4

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)

LAB 4 WRITE-UP

Protocol

Materials

  • Lab coat and disposable gloves
  • PCR reaction mix, 8 tubes, 50 µL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s
  • DNA/ primer mix, 8 tubes, 50 µL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer
  • A strip of empty PCR tubes
  • Disposable pipette
  • Cup for discarded tips
  • Micropipettor
  • OpenPCR machine


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G2 + Positive control none *person tested positive for disease SNP
G2 - Negative control none *person that does not carry the disease SNP
G2 1-1 Patient 1, replicate 1 95353
G2 1-2 Patient 1, replicate 2 95353
G2 1-3 Patient 1, replicate 3 95353
G2 2-1 Patient 2, replicate 1 64816
G2 2-2 Patient 2, replicate 2 64816
G2 2-3 Patient 2, replicate 3 64816


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program

Heated lid: 100°C Initial






Research and Development

PCR - The Underlying Technology






SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing