BME100 f2015:Group8 W1030AM L4

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Materials

  • Lab coat and disposable gloves
  • PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s (http://www.promega.com/resources/protocols/product­information­sheets/g/gotaq­colorless­master­mix­m714-protocol/)
  • DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer
  • A strip of empty PCR tubes
  • Disposable pipette tips: only use each only once. Never re­use disposable pipette tips or samples will be cross ­contaminated
  • Cup for discarded tips
  • Micropipettor
  • OpenPCR machine: shared by two groups