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<!-- パンくずリスト --> <p id="Bread"> <a href=" ">Team Sendai</a> &gt; <a href="">Team Sendai A Top</a>&gt; <a href=" ">Methods</a> </p>

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<h2>AFM</h2> <img src=" " alt="AFM" width="580" height="400"/> <p> <img src=""width="200" height="180"/> &nbsp; AFM enables us to observe nano size structures composed of DNA. Make an observation sample. We put an observation object on a mica for five minutes and wash it in buffer. (Tris/Tris-HCL 20 mM Mg<sup>2+</sup> 12.5 mM pH = 7.4) </p> <h2> Electrophoresis</h2> <p><img src="" alt="AFM" width="580" height="400"/> &nbsp; We can see whether constructing DNA structures is achieved or not by Electrophoresis. It also informs us of DNA length of approximately.</br> &nbsp; The electrophoretic condition is cf. Result & Method.</font></p> <h2> How to create micelle</h2> <p><img src="" alt="soni" "width="400" height="380"/> &nbsp; We dissolve lipid (OcTMAB) in chloroform. Take a small amount of solution, blow-dry it completely with a stream of argon gas. Add TAE Mg<sup>2+</sup> buffer or mQ and multiply the high frequency by ultrasonic liquid processor. </p>


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