BCH4160/2011:Notebook/Jigeshs Lab Notebook 2011/2011/10/20

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Preparing Lipid Films

The purpose of this was to be able to use the lipids prepared for future use.

Materials

  1. CHCl3
  2. MeOH
  3. Lipid vial


Protocol

  1. Make 8mL of 3:1 CHCl3:MeOH (6mL:2mL)
  2. Add 2.5mL of CHCl3:MeOH solution to 1 lipid vial (2 vials total)
  3. Take 1 mL of lipid/CHCl3:MeOH solution and place in 4mL vial (5vials total)
  4. Dry using roto-evaporator
  5. Place vial in desicator overnight to thoroughly dry
  6. Store aliquot in freezer for future use