AU Biomaterials Design Lab:Lab Members/CHEM571:Notebook/Chem 471- Fall 2018/2018/09/05
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08/30/2018-Experimenting with Aliphatic Amino Acids and NaOHOnly Methionine showed a strong shift, though it turned black- no gold nanoparticles but at least it was reactive. This could be because of the sulfur in the R-group. From this we planned a pH assay with both Methionine and Cyestine (which also has a sulfur). For an acidic component: HCl Stock HCl = 36.5% solution --> halved --> 18.25 % --> 9.125% solution --> 2mL stock solution/ 6mL DI water
pH Assay: Acidic-
Control- no pH modifiers Basic-
Two assays: one using Meth one using Cys
4 hours in an 80℃ oven- samples were looked at the following Tuesday (9/11/18) Biggest Changes: Meth 5mM NaOH- deep red Cys 5mM NaOH- Grey/blue precipitated out |