Difference between revisions of "Yu:TEV Cleavage Buffer"

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{| border="0" cellpadding="10" width="100%"
 
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|+'''Solution recipe for 1L TEV Cleavage Buffer.'''
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|+'''Solution recipe for 500 mL TEV Cleavage Buffer.'''
 
|width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker
 
|width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker
 
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|5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder
 
|5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder
|Volume to 1L
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|6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB
 
|6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB

Latest revision as of 10:58, 24 December 2009

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Solution recipe for 500 mL TEV Cleavage Buffer.
1. Measure 100 mL millipore H2O into a 500mL beaker
2. Add 12.5 mL of 1M TRIS-HCl, pH 8.0 25 mM TRIS-HCl
3. Add 15 mL of 5M NaCl 150 mM NaCl
4. Add 500 μL of 0.5M EDTA 0.5 mM EDTA
5. Add 5 mL 10% NP-40 0.1% NP-40
5. Bring volume to 500L with millipore H2O in a graduated cylinder
6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB 1 mM DTT