Difference between revisions of "Yu:TESSX"

From OpenWetWare
Jump to: navigation, search
(No difference)

Latest revision as of 16:03, 1 December 2012

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       UBLogo.jpg

Solution recipe for 100mL TESSX.
1. Measure 70mL H2O into a 100mL beaker.
2. Add 1mL of 1M TRIS (pH 8.0) 10mM TRIS pH 8.0
2. Add 200μL of 0.5M EDTA (pH 8.0) 1mM EDTA pH 8.0
3. Add 2mL of 5M NaCl 100mM NaCl
4. Add 10mL of 10% SDS 1% (w/v) SDS
5. Add 10mL of 20% Triton X-100 2% (v/v) Triton X-100
6. Bring volume to 100mL in a graduated cylinder