Difference between revisions of "Yu:TBS"

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Revision as of 15:57, 9 May 2010

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Solution recipe for 1L 5x Tris-Buffered Saline. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 5x
2. Weigh and add 43.83g NaCl, or add 150mL 5M NaCl 750mM NaCl
3. Add 50mL 1M TRIS-HCl pH 7.5 50mM TRIS pH 7.5
4. Stir until dissolved
5. Bring volume to 1L with millipore water in a graduated cylinder
6. Dilute from 5x to 1x (1:5) for use