Difference between revisions of "Yu:TBS"
Revision as of 16:57, 9 May 2010
|1. Measure 700mL millipore water into a 2L plastic beaker.||5x|
|2. Weigh and add 43.83g NaCl, or add 150mL 5M NaCl||750mM NaCl|
|3. Add 50mL 1M TRIS-HCl pH 7.5||50mM TRIS pH 7.5|
|4. Stir until dissolved|
|5. Bring volume to 1L with millipore water in a graduated cylinder|
|6. Dilute from 5x to 1x (1:5) for use|