Difference between revisions of "Yu:TBE gels"

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(Added entire recipe)
 
m (changed gel volumes)
Line 3: Line 3:
 
{| border="1" cellpadding="10" cellspacing="0"
 
{| border="1" cellpadding="10" cellspacing="0"
 
|width="200px" valign="top"|
 
|width="200px" valign="top"|
|width="100px" valign="top"|'''15 mL'''
+
|width="100px" valign="top"|'''20 mL'''
|width="100px" valign="top"|'''30 mL'''
+
|width="100px" valign="top"|'''40 mL'''
|width="100px" valign="top"|'''45 mL'''
 
 
|width="100px" valign="top"|'''60 mL'''
 
|width="100px" valign="top"|'''60 mL'''
 +
|width="100px" valign="top"|'''80 mL'''
 
|-
 
|-
 
|Protogel
 
|Protogel
|4.0 mL
+
|5.3 mL
|8.0 mL
+
|10.6 mL
|12.0 mL
+
|15.9 mL
|16.0 mL
+
|21.2 mL
 
|-
 
|-
 
|milliQ H<sub>2</sub>O
 
|milliQ H<sub>2</sub>O
|7.9 mL
+
|10.5 mL
|15.8 mL
+
|21 mL
|23.7 mL
 
 
|31.5 mL
 
|31.5 mL
 +
|42 mL
 
|-
 
|-
 
|5x [[Yu:TBE|TBE]]
 
|5x [[Yu:TBE|TBE]]
|3.0 mL
+
|4.0 mL
|6.0 mL
+
|8.0 mL
|9.0 mL
 
 
|12.0 mL
 
|12.0 mL
 +
|16.0 mL
 
|-
 
|-
 
|TEMED
 
|TEMED
|5.25 μL
+
|14.0 μL
|10.5 μL
+
|28.0 μL
|15.75 μL
+
|42.0 μL
|21.0 μL
+
|56.0 μL
 
|-
 
|-
 
|10% APS
 
|10% APS
|105 μL
+
|140 μL
|210 μL
+
|280 μL
|315 μL
 
 
|420 μL
 
|420 μL
 +
|560 μL
 
|-
 
|-
 
|}
 
|}

Revision as of 07:08, 4 November 2010

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20 mL 40 mL 60 mL 80 mL
Protogel 5.3 mL 10.6 mL 15.9 mL 21.2 mL
milliQ H2O 10.5 mL 21 mL 31.5 mL 42 mL
5x TBE 4.0 mL 8.0 mL 12.0 mL 16.0 mL
TEMED 14.0 μL 28.0 μL 42.0 μL 56.0 μL
10% APS 140 μL 280 μL 420 μL 560 μL