Yu:STET: Difference between revisions
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(STET) |
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Latest revision as of 16:58, 1 December 2012
1. Measure 50mL H2O into a 100mL beaker. | |
2. Add 8g sucrose | 8% (w/v) Sucrose |
3. Add 5mL of 1M TRIS (pH 8.0) | 50mM TRIS pH 8.0 |
4. Add 10mL of 0.5M EDTA (pH 8.0) | 50mM EDTA pH 8.0 |
5. Add 25mL of 20% Triton X-100 | 5% Triton X-100 |
6. Bring volume to 100mL in a graduated cylinder | |
7. Sterilize by filtration. |