Difference between revisions of "Yu:RNAse"

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[[Category:Yu Solutions]]

Latest revision as of 14:22, 22 September 2009

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Solution recipe for RNAse(10mg/ml).
Per 50ml:
1. 50ml MiliQ water
2. 0.5g RNAseA
3. 0.5ml TRis HCl(1M stock, pH 7.5)(10mM final)
4.0.0438g NaCl(15mM final)
5. Mix, heat to boil for 10 mins, filter sterilize (0.2μL)
6.Make 1 ml aliquots,store at -200C