Yu:RNAse: Difference between revisions
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|6.Make 1 ml aliquots,store at -20<sup>0</sup>C | |6.Make 1 ml aliquots,store at -20<sup>0</sup>C | ||
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[[Category:Yu Solutions]] |
Latest revision as of 14:22, 22 September 2009
Per 50ml: |
1. 50ml MiliQ water |
2. 0.5g RNAseA |
3. 0.5ml TRis HCl(1M stock, pH 7.5)(10mM final) |
4.0.0438g NaCl(15mM final) |
5. Mix, heat to boil for 10 mins, filter sterilize (0.2μL) |
6.Make 1 ml aliquots,store at -200C |