Yu:Procedures: Difference between revisions
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{|width="100%" border="0" cellpadding="10" | {|width="100%" border="0" cellpadding="10" | ||
|width="30%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"> | |width="30%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Solutions</font></div> | ||
*[[Yu:YEPD|YEPD]] | *[[Yu:YEPD|YEPD]] | ||
*[[Yu:SOC|SOC]] | |||
*[[Yu:Dropout Media|Dropout Media]] | *[[Yu:Dropout Media|Dropout Media]] | ||
*[[Yu:Lysis | *[[Yu:Lysis#buffer|Lysis Buffer]] | ||
*[[Yu:SDS_PAGE Buffer|SDS-PAGE Buffer]] | *[[Yu:SDS_PAGE Buffer|SDS-PAGE Buffer]] | ||
*[[Yu:Western Transfer Buffer| | *[[Yu:Western Transfer Buffer|Transfer Buffer for Western Blot]] | ||
*[[Yu:PBS|PBS]] | *[[Yu:PBS|PBS]] | ||
**[[Yu:PBS#PBST|PBST]] | **[[Yu:PBS#PBST|PBST]] | ||
**[[Yu:PBS#PBSMT|PBSMT]] | **[[Yu:PBS#PBSMT|PBSMT]] | ||
*[[Yu:TAE|TAE]] | *[[Yu:TBS|TBS]] | ||
**[[Yu:TBS#TBS-TT|TBS/TT]] | |||
*[[Yu:TAE|50x TAE]] | |||
*[[Yu:TBE|10x TBE]] | |||
*[[Yu:LiOAc|LiOAc/TE]] | |||
*[[Yu:IPP|IPP150]] | |||
**[[Yu:IPP#IPP300|IPP300]] | |||
*[[Yu:NP40 Buffer|NP-40 Buffer]] | |||
*[[Yu:TEV Cleavage Buffer|TEV Cleavage Buffer]] | |||
*[[Yu:CBB|Calmodulin-Binding Buffer]] | |||
*[[Yu:TES|TES]] | |||
*[[Yu:Super_SPO|Super SPO Media]] | |||
*[[Yu:P Solution|P Solution]] | |||
|valign="top" align="left"| | |width="70%" valign="top" align="left"| | ||
{|border="0" cellpadding="10" | {|border="0" cellpadding="10" | ||
|width=" | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top">[[Yu:Western|<font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Western Blot</font>]]</div> | ||
*[[Yu: | *[[Yu:Lysis|Lysing Cells]] | ||
*[[Yu: | *[[Yu:SDS_PAGE|SDS-PAGE Gel]] | ||
*[[Yu:Western#Transfer|Western Transfer]] | *[[Yu:Western#Transfer|Western Transfer]] | ||
*[[Yu:Western#Immunoblotting|Immunoblotting]] | *[[Yu:Western#Immunoblotting|Immunoblotting]] | ||
*[[Yu:Western#Developing|Developing]] | *[[Yu:Western#Developing|Developing]] | ||
*[[Yu:Western#Stripping|Stripping]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Transformation</font></div> | |||
*[[Yu:Transformation|Yeast LiOAc Transformation]] | |||
*[[Yu:e_coli_transform|''E. coli'' Transformation]] | |||
*[[Yu:Competent_Cells|''E. coli'' Normal Competent Cells]] | |||
*[[Yu:quick_miniprep|Quick E. coli plasmid mini prep]] | |||
*[[Yu:Yeast_miniprep|Yeast Plasmid Miniprep]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Southern Blot</font></div> | |||
*[[Yu:southern_sol|Solutions]] | |||
*[[Yu:Southern_Blotting|Southern Blotting]] | |||
|- | |- | ||
|width=" | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">CoIP</font></div> | ||
*[[Yu:CoIP|TAP CoIP]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">PCR</font></div> | |||
| | *[[Yu:homemade_taq|Homemade Taq]] | ||
*[[Yu:ChIP_PCR|ChIP PCR (qPCR)]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Spheroplast Preperations</font></div> | |||
*[[Yu:sphero_sol|Solutions]] | |||
*[[Yu:spheroplast|Spheroplast preperation]] | |||
*[[Yu:nuclei|Nuclei Isolation]] | |||
|- | |- | ||
|width=" | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">ChIP</font></div> | ||
*[[Yu:TBE_gels|8% TBE polyacrylamide gel]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Northern Blot</font></div> | |||
*[[Yu:RNA_extract|RNA extraction]] | |||
*[[Yu:Agarose_Formaldehyde|Agarose-Formaldehyde gels]] | |||
*[[Yu:High-resolution_gels|High-resolution gels]] | |||
*[[Yu:Northern_solns|Northern Solutions]] | |||
*[[Yu:PNK_label|Oligo End Labeling]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Chromatin mapping</font></div> | |||
*[[Yu:MNase_dig|MNase digestion]] | |||
*[[Yu:probe_design|Probe Design]] | |||
|- | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Cloning</font></div> | |||
*[http://tools.invitrogen.com/content/sfs/manuals/topota_man.pdf TOPO TA Manual] | |||
*[[Yu:Genomic_prep|Rapid Genomic Prep]] | |||
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Misc</font></div> | |||
*[[Yu:Dialysis|Dialysis]] | |||
|} | |} | ||
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|} | |} | ||
Revision as of 16:59, 1 December 2012
Solutions
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