Difference between revisions of "Yu:PLAAC"

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|+'''Solution recipe for PLAAC(Protease inhibitor cocktail for yeast)'''.
 
|+'''Solution recipe for PLAAC(Protease inhibitor cocktail for yeast)'''.
 
|-
 
|-
| 1:1:1:1 of lab stock=1000x
+
| 3ng/μL Pepstatin
 
|-
 
|-
| Can add PMSF(1mM final)
+
| 3ng/μL Leupeptin
 
|-
 
|-
| Final concentration =3ng/μL of Pepstatin, Leu, Apro, Chy, Antipain
+
| 3ng/μL Antipain
 
|-
 
|-
 +
| 3ng/μL Aprotinin
 +
|-
 +
| 3ng/μL Chymostatin
 +
|-
 +
|Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.
 +
|}
  
 
[[Category:Yu Solutions]]
 
[[Category:Yu Solutions]]

Revision as of 07:15, 1 September 2011

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Solution recipe for PLAAC(Protease inhibitor cocktail for yeast).
3ng/μL Pepstatin
3ng/μL Leupeptin
3ng/μL Antipain
3ng/μL Aprotinin
3ng/μL Chymostatin
Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.