Difference between revisions of "Yu:PLAAC"

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PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.
 
PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.
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|+'''Solution recipe for PLAAC Stock'''.
 
|+'''Solution recipe for PLAAC Stock'''.

Latest revision as of 06:18, 1 September 2011

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PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.

Solution recipe for PLAAC Stock.
3ng/μL Pepstatin
3ng/μL Leupeptin
3ng/μL Antipain
3ng/μL Aprotinin
3ng/μL Chymostatin
Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.