Yu:PLAAC: Difference between revisions
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PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled. | PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled. | ||
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|+'''Solution recipe for PLAAC Stock'''. | |+'''Solution recipe for PLAAC Stock'''. | ||
Latest revision as of 07:18, 1 September 2011
PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.
| 3ng/μL Pepstatin |
| 3ng/μL Leupeptin |
| 3ng/μL Antipain |
| 3ng/μL Aprotinin |
| 3ng/μL Chymostatin |
| Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C. |
