Yu:PBS: Difference between revisions
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m (fix) |
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{| border="0" cellpadding="10" | {| border="0" cellpadding="10" | ||
|+'''Solution recipe for 1L 1x <span id="PBSMT">PBSMT</span>'''. | |+'''Solution recipe for 1L 1x <span id="PBSMT">PBSMT</span>'''. | ||
|width="600px" valign="top"|1. Measure | |width="600px" valign="top"|1. Measure 100mL 10x PBS into a beaker. | ||
|width="100px" valign="top"| | |width="100px" valign="top"|1x [[Yu:PBS#PBS|PBS]] | ||
|- | |- | ||
|2. Add 3mL 1M KCl | |2. Add 3mL 1M KCl | ||
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|0.5% [[Triton X-100]] | |0.5% [[Triton X-100]] | ||
|- | |- | ||
|5. | |5. Bring to 1L volume with H<sub>2</sub>O and stir until mixed | ||
| | | | ||
|} | |} | ||
[[Category:Yu Solutions]] | [[Category:Yu Solutions]] |
Latest revision as of 15:41, 14 May 2010
1. Measure 700mL millipore water into a 2L plastic beaker. | 10x |
2. Weigh 80g NaCl and add it to the mixture | 1.4M NaCl |
5. Weigh 2g KCl and add it to the mixture | 30mM KCl |
4. Weigh 14.4g Na2HPO4 and add it to the mixture | 100mM Na2HPO4 |
5. Weigh 2.4g KH2PO4 and add it to the mixture | 20mM KH2PO4 |
6. Stir until dissolved | |
7. Bring volume to 1L with millipore water in a graduated cylinder | |
8. Dilute from 10x to 1x (1:10) for use |
1. Measure 1L 1x PBS into a 1L bottle. | 1L 1x PBS |
2. Pipette 2.5mL TWEEN 20 into the bottle | 0.25% TWEEN 20 |
3. Stir until mixed |
1. Measure 100mL 10x PBS into a beaker. | 1x PBS |
2. Add 3mL 1M KCl | 3mM KCl |
3. Add 2.5mL 1M MgCl2 | 2.5mM MgCl2 |
4. Add 5mL Triton X-100 | 0.5% Triton X-100 |
5. Bring to 1L volume with H2O and stir until mixed |