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Solution recipe for 100mL 5x LiOAc/TE. Dilute to 1x for use.
1. Measure 50mL 1M LiOAC into a 250mL beaker to bring solution to .5M LiOAc 50mL 1M LiOAC
2. Add 5mL 1M Tris pH 8.0 to bring solution to .05M Tris 5mL 1M Tris pH 8.0
3. Add 2mL .5M EDTA to bring solution to .01M EDTA 2mL .5M EDTA
4. Bring volume to 100mL by adding 43mL sterile H2O Volume to 100mL with 43mL H2O
5. Sterilize solution with sterile filtering technique Sterile filter