Yu:LiOAc: Difference between revisions

Latest revision as of 14:12, 4 May 2010

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**Solution recipe for 100mL 5x LiOAc/TE. Dilute to 1x for use.**
1. Measure 50mL 1M LiOAC into a 250mL beaker	0.5M LiOAc
2. Add 5mL 1M Tris pH 8.0	50mM TRIS
3. Add 2mL .5M EDTA pH 8.0	10mM EDTA
4. Bring volume to 100mL by adding 43mL sterile H₂O
5. Sterilize solution with sterile filtering technique

@@ Line 3: / Line 3: @@
 {| border="0" cellpadding="10"
 |+'''Solution recipe for 100mL 5x <span id="TE">LiOAc/TE</span>. Dilute to 1x for use.'''
-|width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker to bring solution to .5M LiOAc
+|width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker
-|width="100px" valign="top"|50mL 1M LiOAC
+|width="100px" valign="top"|0.5M LiOAc
 |-
-|2. Add 5mL 1M [[Tris]] pH 8.0 to bring solution to .05M Tris
+|2. Add 5mL 1M [[Tris]] pH 8.0
-|5mL 1M Tris pH 8.0
+|50mM TRIS
 |-
-|3. Add 2mL .5M [[EDTA]] to bring solution to .01M EDTA
+|3. Add 2mL .5M [[EDTA]] pH 8.0
-|2mL .5M EDTA
+|10mM EDTA
 |-
 |4. Bring volume to 100mL by adding 43mL sterile H<sub>2</sub>O
-|Volume to 100mL with 43mL H<sub>2</sub>O
+|
 |-
 |5. Sterilize solution with sterile filtering technique
-|Sterile filter
+|
 |}