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Solution recipe for IPTG(Powder stored at -200C).
1. Dissolve a solution of IPTG of 2g of IPTG in 8ml of milipore water H2O
2. Adjust the volume of the solution to 10ml with milipore H2O
3. Sterilize by filtration through a 0.22 micron disposable filter
4. Dispense the solution into 1-ml aliquots and store them at -200C