Yu:IPP
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1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0 | 12.5mL 1M Tris pH 8.0 |
2. Add 15mL of 5M NaCl for a final concentration of 150mM NaCl | 15mL 5M NaCl |
3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40 | 5mL 10% NP-40 |
4. Bring volume to 500mL with millipore H2O in a graduated cylinder | Volume to 500mL |
1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0 | 12.5mL 1M Tris pH 8.0 |
2. Add 30mL of 5M NaCl for a final concentration of 300mM NaCl | 30mL 5M NaCl |
3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40 | 5mL 10% NP-40 |
4. Bring volume to 500mL with millipore H2O in a graduated cylinder | Volume to 500mL |