Difference between revisions of "Yu:FORMAMIDE"

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(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for FORMAMIDE LOADING BUFFER FOR ACYLAMIDE GEL'''. |- | 0.05%(w/v)bromophenol blue, |- | 0.05%(w/v) xylene cyanol |- |...)
 
 
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{{Template:Yu}}
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#REDIRECT [[Yu:Northern_solns#Formamide_Loading_Buffer]]
 
 
{| border="0" cellpadding="10"
 
|+'''Solution recipe for FORMAMIDE LOADING BUFFER FOR ACYLAMIDE GEL'''.
 
|-
 
| 0.05%(w/v)bromophenol blue,
 
|-
 
| 0.05%(w/v) xylene cyanol
 
|-
 
| 20mM EDTA
 
|-
 
| For 10 ml:
 
|-
 
|1.  0.005g BP
 
|-
 
|2.  0.005g xc
 
|-
 
|3.  400ul 0.5M EDTA
 
|-
 
|4.  9.6 ml Formamide(best is Fluka)
 
|-
 
|5.  -20<sup>0</sup>C storage. w/foild, use aliquots in eppis
 

Latest revision as of 14:05, 22 September 2009