YT

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Revision as of 00:53, 28 June 2012 by Christopher C Vanlang (talk | contribs) (New page: == Summary == 2x YT Medium. == Ingredients == ===For 1000 ml=== *10g Bacto yeast extract *16g Bacto Tryptone *5g NaCl Adjust to pH 7.0 prior to use. ==Extended instructions== *combine:...)
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Summary

2x YT Medium.

Ingredients

For 1000 ml

  • 10g Bacto yeast extract
  • 16g Bacto Tryptone
  • 5g NaCl

Adjust to pH 7.0 prior to use.

Extended instructions

  • combine:
Reagent for 1 L 500 mL 100 mL
tryptone 16 g 8 g 1.6 g
yeast 10 g 5 g 1 g
NaCl 5 g 2.5 g 0.5 g
ddH2O 980 ml 490 ml 98 ml
  • adjust pH to 7.0 w/ NaOH
  • bring to volume
  • Sterilize by autoclaving

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia