Difference between revisions of "Xylene cyanol"

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'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel (while it's own molecular weight is around 540g/mol).
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[[Image:Agarose gel dyes.jpg|right|200px]]
  
* 5000 bp at 0.5
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'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).
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* 10'000 bp at 0.7%
 
* 4000 bp at 1.5%
 
* 4000 bp at 1.5%
 
* 750 bp at 2.0%
 
* 750 bp at 2.0%
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== see also ==
 
== see also ==
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* [[agarose gel loading buffer]]
 
* [[agarose gel electrophoresis]]
 
* [[agarose gel electrophoresis]]
* [[Cresol Red]]
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* [[Cresol Red]] - faster than XC
* [[Bromophenol blue]]
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* [[Bromophenol blue]] - faster than XC
  
 
== external links ==
 
== external links ==
 
* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia]
 
* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia]
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[[Category:Material]] [[Category:Agarose gel electrophoresis]] [[Category:Dye]]

Latest revision as of 06:20, 3 June 2009

Agarose gel dyes.jpg

Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than bromophenol blue (670g/mol) because of its charge (1 net negative charge).

  • 10'000 bp at 0.7%
  • 4000 bp at 1.5%
  • 750 bp at 2.0%
  • 200 bp at 3.0%

order information

  • CAS 2650-17-1

see also

external links