Difference between revisions of "X-Gal"

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(New page: X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commo...)
 
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X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening.  
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X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues.  
  
A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml.
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A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation.
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==Protocols using X-Gal==
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*[[LacZ staining of cells]]
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==Notes==
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*Also may make stock solution with 50 mg in about 250 µl DMSO
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==See also==
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*[[Beta-galactosidase assay]]
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==External links==
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* S-Gal - a variation of X-Gal [http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Life_Science_Innovations/April_2001/April_2001_Feature_Article.html]
  
 
[[Category:Material]] [[Category:Chemical]]
 
[[Category:Material]] [[Category:Chemical]]

Latest revision as of 14:22, 23 December 2011

X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues.

A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation.

Protocols using X-Gal

Notes

  • Also may make stock solution with 50 mg in about 250 µl DMSO

See also

External links

  • S-Gal - a variation of X-Gal [1]