Difference between revisions of "Wittrup:Protocols"

From OpenWetWare
Jump to: navigation, search
 
Line 3: Line 3:
 
[[Wittrup:Buffers]]
 
[[Wittrup:Buffers]]
  
[[Wittrup:Amino Acids]]
+
[[Wittrup:Yeast media]]
  
[[Wittrup:Yeast media]]
+
===Assays===
  
===Yeast Protocols===
 
  
===Strains/Storage===
+
===DNA===
  
===Assays===
+
===RNA===
  
 
===Proteins===
 
===Proteins===
  
===DNA===
+
[[Wittrup: Determining Protein Concentration]]
 +
 
 +
[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]]
 +
 
 +
[[Wittrup:FPLC general use]]
 +
 
 +
[[Wittrup:SDS-PAGE]]
 +
 
 +
[[Wittrup:ECL Western Blots and Phosphorimager]]
 +
 
 +
[[Wittrup:Protein concentration by ultrafiltration]]
 +
 
 +
 
 +
===Yeast Protocols===
 +
 
 +
[[Wittrup:  Yeast Transformation]]
  
===RNA===
+
[[Wittrup:  Plasmid rescue from yeast]]
  
===Software===
+
[[Wittrup:  Yeast storage and revival]]
  
 +
[[Wittrup:  Yeast surface display]]
  
===E. coli libraries===
+
===Cell culture===
  
===Yeast surface display===
+
===Software===

Revision as of 16:35, 15 January 2006

Buffers/Equipment/Media

Wittrup:Buffers

Wittrup:Yeast media

Assays

DNA

RNA

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use

Wittrup:SDS-PAGE

Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration


Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Software