Wittrup:Protocols: Difference between revisions
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===General Lab Duties=== | |||
[[Wittrup: Dishwashing]] | |||
===Recipes=== | ===Recipes=== | ||
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[[Wittrup: PCR]] | [[Wittrup: PCR]] | ||
[[Wittrup: Yeast colony PCR]] | |||
===Proteins=== | ===Proteins=== | ||
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[[Wittrup: TCA Protein Precipitation]] | [[Wittrup: TCA Protein Precipitation]] | ||
[[Wittrup: TEV Protease Purification and Digestion]] | |||
===Yeast Protocols=== | ===Yeast Protocols=== | ||
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===Cell culture=== | ===Cell culture=== | ||
[[Wittrup: New Cell Line Banking and Authentication]] | |||
[[Wittrup: Basic Culture Media]] | [[Wittrup: Basic Culture Media]] | ||
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[[Wittrup: Thawing Protocol]] | [[Wittrup: Thawing Protocol]] | ||
[[Wittrup: Crystal Violet Toxicity Protocol]] | [[Wittrup: Crystal Violet Toxicity Assay Protocol]] | ||
[[Wittrup: HEK293-F Transfection]] | |||
[[Wittrup: Mycoplasma Detection and Clearance]] | |||
===Animal studies=== | |||
[[Wittrup:Immunohistochemistry of frozen sections]] | |||
[[Wittrup:Immunohistochemistry of paraffin embedded sections]] | |||
[[Wittrup:Immunofluorescence of frozen sections]] | |||
[[Wittrup:Tumor dissociation]] | |||
[[Wittrup:Making a cell line from tumor sections]] | |||
===Individual Lab Member Protocols=== | |||
[[User: Cary F Opel]] | |||
Revision as of 10:59, 16 August 2012
General Lab Duties
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Restriction digestion
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup: ECL Western Blots and Phosphorimager
Wittrup: Protein concentration by ultrafiltration
Wittrup: Protein conjugation with fluorophores
Wittrup: Fluorescein Quench Titration Assay
Wittrup: TCA Protein Precipitation
Wittrup: TEV Protease Purification and Digestion
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Wittrup: Cell Surface Secretion Assay
Wittrup: Fluorescence Microscopy of Yeast
Wittrup: Yeast Preps for Western Blotting
Cell culture
Wittrup: New Cell Line Banking and Authentication
Wittrup: Subculturing Protocol
Wittrup: Crystal Violet Toxicity Assay Protocol
Wittrup: HEK293-F Transfection
Wittrup: Mycoplasma Detection and Clearance
Animal studies
Wittrup:Immunohistochemistry of frozen sections
Wittrup:Immunohistochemistry of paraffin embedded sections
Wittrup:Immunofluorescence of frozen sections
Wittrup:Making a cell line from tumor sections
