Difference between revisions of "Wittrup:Protocols"

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[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]]
 
[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]]
  
[[Wittrup:FPLC general use]]
+
[[Wittrup: FPLC general use]]
  
[[Wittrup:SDS-PAGE]]
+
[[Wittrup: SDS-PAGE]]
  
[[Wittrup:ECL Western Blots and Phosphorimager]]
+
[[Wittrup: ECL Western Blots and Phosphorimager]]
  
[[Wittrup:Protein concentration by ultrafiltration]]
+
[[Wittrup: Protein concentration by ultrafiltration]]
  
[[Wittrup:Protein conjugation with fluorophores]]
+
[[Wittrup: Protein conjugation with fluorophores]]
 +
 
 +
[[Wittrup:  Fluorescein Quench Titration Assay]]
 +
 
 +
[[Wittrup:  TCA Protein Precipitation]]
  
 
===Yeast Protocols===
 
===Yeast Protocols===
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[[Wittrup:  Yeast surface display]]
 
[[Wittrup:  Yeast surface display]]
  
 +
[[Wittrup:  Cell Surface Secretion Assay]]
 +
 +
[[Wittrup:  Fluorescence Microscopy of Yeast]]
 
===Cell culture===
 
===Cell culture===
  

Revision as of 11:26, 24 July 2006

Recipes

Wittrup: Buffers

Wittrup: Yeast Media/Buffers

Wittrup: E. coli Media

Equipment

Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope

DNA

Wittrup: Plasmid miniprep

Wittrup: Restriction digestion

Wittrup: Gel electrophoresis

Wittrup: Gel extraction

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup: FPLC general use

Wittrup: SDS-PAGE

Wittrup: ECL Western Blots and Phosphorimager

Wittrup: Protein concentration by ultrafiltration

Wittrup: Protein conjugation with fluorophores

Wittrup: Fluorescein Quench Titration Assay

Wittrup: TCA Protein Precipitation

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Wittrup: Cell Surface Secretion Assay

Wittrup: Fluorescence Microscopy of Yeast

Cell culture

Wittrup: Basic Culture Media

Wittrup: Recovery Media

Wittrup: Freezing Media

Wittrup: Freezing Protocol

Wittrup: Subculturing Protocol

Wittrup: Cell Counting Protocol

Wittrup: Thawing Protocol

Software

Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench