Difference between revisions of "Wittrup:Protocols"

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[[Wittrup: Freezing Media]]
[[Wittrup: Freezing Media]]
[[Wittrup: Freezing Protocol]]
[[Wittrup: Subculturing Protocol]]
[[Wittrup: Cell Counting Protocol]]
[[Wittrup: Thawing Protocol]]

Revision as of 19:49, 25 March 2006


Wittrup: Buffers

Wittrup: Yeast Media/Buffers

Wittrup: E. coli Media


Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope


Wittrup: Plasmid miniprep

Wittrup: Restriction digestion

Wittrup: Gel electrophoresis

Wittrup: Gel extraction

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR


Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use


Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration

Wittrup:Protein conjugation with fluorophores

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Wittrup: Basic Culture Media

Wittrup: Recovery Media

Wittrup: Freezing Media

Wittrup: Freezing Protocol

Wittrup: Subculturing Protocol

Wittrup: Cell Counting Protocol

Wittrup: Thawing Protocol


Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench