Difference between revisions of "Wittrup:Protocols"

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===Recipes===
 
===Recipes===
  
[[Wittrup:Buffers and media]]
+
[[Wittrup: Buffers]]
 +
 
 +
[[Wittrup: Yeast Media/Buffers]]
 +
 
 +
[[Wittrup: E. coli Media]]
  
 
===Equipment===
 
===Equipment===

Revision as of 11:12, 10 February 2006

Recipes

Wittrup: Buffers

Wittrup: Yeast Media/Buffers

Wittrup: E. coli Media

Equipment

Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope

DNA

Wittrup: Plasmid minipreps, restriction digestion

Wittrup: Gel electrophoresis and band purification

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use

Wittrup:SDS-PAGE

Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration

Wittrup:Protein conjugation with fluorophores

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Wittrup: Cell culture protocols

Software

Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench