Difference between revisions of "Wikiomics:PSI-BLAST"

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* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html  @NCBI Part 3]
 
* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html  @NCBI Part 3]
 
* [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]]
 
* [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]]
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[[Category:Protocol]] [[Category:In silico]] [[Category:Sequence analysis]]

Latest revision as of 14:35, 3 March 2008

PSI-BLAST - Position-Specific Iterated BLAST. Variant of BLAST used for disovery of weak but relevant protein sequence matches. The algorithm is based on running consequitive BLAST searches using position weight matices:

  • query sequence ->
  • first blast search ->
  • first iteration blast results ->
  • sequence alignment of strong matches ->
  • creating a profile #1 ->
  • second iteration blast search with profile #1 as a query ->
  • second iteration blast results, etc.

PSI-BLAST server at NCBI: link

Tutorials