Wikiomics:PSI-BLAST: Difference between revisions
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* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html @NCBI Part 3] | * [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html @NCBI Part 3] | ||
* [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]] | * [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]] | ||
[[Category:Protocol]] [[Category:In silico]] [[Category:Sequence analysis]] | |||
Latest revision as of 14:35, 3 March 2008
PSI-BLAST - Position-Specific Iterated BLAST. Variant of BLAST used for disovery of weak but relevant protein sequence matches. The algorithm is based on running consequitive BLAST searches using position weight matices:
- query sequence ->
- first blast search ->
- first iteration blast results ->
- sequence alignment of strong matches ->
- creating a profile #1 ->
- second iteration blast search with profile #1 as a query ->
- second iteration blast results, etc.
PSI-BLAST server at NCBI: link
