WangLab:Measure DNA Concentration

From OpenWetWare
Revision as of 08:02, 11 September 2007 by Lu6007 (talk | contribs) (New page: 1. Choose DNA protocol -> enter, enter, enter... 2. Choose Dilution Factor Usually 20x for miniprep product, and 100x for maxprep product. 3. Subtract Background Blank H2O 200 100 ul...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

1. Choose DNA protocol -> enter, enter, enter... 2. Choose Dilution Factor

  Usually 20x for miniprep product, and 100x for maxprep product.

3. Subtract Background

  Blank H2O 200 100 ul (miniprep, 200 ul for maxiprep)
  -> Read blank

4. Continue -> Read Sample -> Measure twice -> Read sample -> ?

  The amount of DNA needed:
  miniprep: 5 ul dilute 20 times -> 100 ul
  maxiprep: 2 ul dilute 100 times -> 200 ul ; 
  and measure again after adding water to adjust concentration.