Waldminghaus:Back Door/Standard Solutions
From OpenWetWare
The following solutions are good to have available because they are used in many recipes as standard:
Name | Usual size |
---|---|
5M NaCl | 200 ml |
0.5M EDTA | 100 ml |
AB Media
Vol | material | Final concentration |
---|---|---|
200 ml | 5xA | 1x |
400 ml | 2xB | 1x |
10 ml | 20% Glucose | 0,2% |
20 ml | 20% Sodium-Acetate | 0,4% |
50 ml | 10% CAA | 0,5% |
1 ml | 10 mg/ml Thiamine | 10 µg/ml |
2,5 ml | 10 mg/ml Uridine | 25 µg/ml |
Fill up to one liter with sterile H2O!
You have to choose your carbonesource between Soduim-Acetate, Glucose or Glucose and CAA.