Difference between revisions of "Waldminghaus:Back Door/Standard Solutions"

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(New page: {{Template:Waldminghaus}} '''The following solutions are good to have available because they are used in many recipes as standard:''' {| border="3" ! Name!! Usual size |- | 5M NaCl || 20...)
 
(AB Media)
 
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== AB Media ==
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{|border="1"
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!Vol !!material !!Final concentration
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|-
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|200 ml
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|5xA
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|1x
 +
|-
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|400 ml
 +
|2xB
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|1x
 +
|-
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|10 ml
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|'''20% Glucose'''
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|0,2%
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|-
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|20 ml
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|''' 20% Sodium-Acetate'''
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|0,4%
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|-
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|50 ml
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|'''10% CAA'''
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|0,5%
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|-
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|1 ml
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|10 mg/ml Thiamine
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|10 µg/ml
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|-
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|2,5 ml
 +
|10 mg/ml Uridine
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|25 µg/ml
 +
|}
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Fill up to one liter with sterile H<sub>2</sub>O!<br>
 +
You have to choose your carbonesource between Soduim-Acetate, Glucose or Glucose and CAA.

Latest revision as of 10:43, 11 March 2014

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The following solutions are good to have available because they are used in many recipes as standard:

Name Usual size
5M NaCl 200 ml
0.5M EDTA 100 ml


AB Media

Vol material Final concentration
200 ml 5xA 1x
400 ml 2xB 1x
10 ml 20% Glucose 0,2%
20 ml 20% Sodium-Acetate 0,4%
50 ml 10% CAA 0,5%
1 ml 10 mg/ml Thiamine 10 µg/ml
2,5 ml 10 mg/ml Uridine 25 µg/ml

Fill up to one liter with sterile H2O!
You have to choose your carbonesource between Soduim-Acetate, Glucose or Glucose and CAA.