Van Oudenaarden Lab:T01D3.2: Difference between revisions

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gaattgaggaaacaaatgtg, hlh-34_40, 40, 945, 35
gaattgaggaaacaaatgtg, hlh-34_40, 40, 945, 35
----------------------------------------------------------------------
Input sequence with probe locations:
atggagactaatctttctgaagaaaaacaaaaaccgtcaaaatcacaagcccagcagagaagacaaatgg
  cctctgattagaaagacttc        ttggcagttttagtgttcgg  cgtctcttctgtttacc
  Probe # 1, 40% GC            Probe # 2, 45% GC    Probe # 3, 40% GC
aaaattatgaattctcacagctagccaatgaacttccattggcaagagcaatttctggacagcatattga
ttt  tacttaagagtgtcgatcgg  acttgaaggtaaccgttctc  taaagacctgtcgtataact
      Probe # 4, 45% GC    Probe # 5, 45% GC    Probe # 6, 35% GC 
taaaacaacgatggttagattagcaactgcttatatcaaacttcacaatatatttgggcaatctcaaaga
    tgttgctaccaatctaatcg  gacgaatatagtttgaagtg  tataaacccgttagagtttc
    Probe # 7, 40% GC    Probe # 8, 35% GC      Probe # 9, 35% GC   
gcctacagttcagctgactattattatggaagtgactctttatggactaacaatcatttagatctactag
ggatgtcaagtcgactgata      ccttcactgagaaatacctg      gtaaatctagatgatc
Probe # 10, 45% GC        Probe # 11, 45% GC        Probe # 12, 35%
atggattctttgtgatactggacaggcgcggagatgtactttacataagtgaaactattagtatttactt
tacc  agaaacactatgacctgtcc  gcctctacatgaaatgtatt      gataatcataaatgaa
GC    Probe # 13, 45% GC    Probe # 14, 35% GC        Probe # 15, 35%
gggcttgtcacaagttgaaatgactggcaacgcgatggttgattatattcatgaacaggatatcaattgc
cccg    gtgttcaactttactgaccg  gcgctaccaactaatataag  cttgtcctatagttaacg
GC      Probe # 16, 45% GC    Probe # 17, 40% GC    Probe # 18, 35% GC
tttaactcggcccttaactattgtgaccttaattggcctcaaatgtgcaacgtacgtgttaaatctagtc
aa  tgagccgggaattgataaca  ggaattaaccggagtttaca    gcatgcacaatttagatcag
    Probe # 19, 45% GC    Probe # 20, 40% GC      Probe # 21, 40% GC 
tcacaaagcgagcaaacaaggatgcagttcgtgcttcacctggatacaaggttcttcgtctagaaattac
  tgtttcgctcgtttgttcct  gtcaagcacgaagtggacct  gttccaagaagcagatcttt  tg
  Probe # 22, 45% GC    Probe # 23, 55% GC    Probe # 24, 40% GC    Pr
aatgggtccaaatacaaatactagaatgattgcatgttatccaatgcctactccagttctttctacagta
ttacccaggtttatgttt    tcttactaacgtacaatagg  acggatgaggtcaagaaaga  cat
obe # 25, 35% GC      Probe # 26, 35% GC    Probe # 27, 45% GC    Pro
accattccttctaattcatttgttatcattacatccatcgatcttcatataacatttgctgatgaaaaag
tggtaaggaagattaag        gtaatgtaggtagctagaag          acgactactttttc
be # 28, 35% GC          Probe # 29, 40% GC            Probe # 30, 40
ctcatcagttgcttaataacccattttacccagattcaaatatcaaaggaatgtcattatattcactgat
gagtag          ttgggtaaaatgggtctaag        tccttacagtaatataagtg   
% GC            Probe # 31, 40% GC          Probe # 32, 30% GC     
tgatatctcagattctgaagttatatcaaaaatgcatttcgatattttcaacctgggcgcttataaaacc
actatagagtctaagacttc            cgtaaagctataaaagttgg    gcgaatattttgg
Probe # 33, 35% GC              Probe # 34, 35% GC      Probe # 35, 4
ccgtactatcgaatgatcttgaatcaaacaagtgaaactttttacgtcgagtcaaatatttttcgtcata
ggcatga  gcttactagaacttagtttg  cactttgaaaaatgcagctc        aaagcagtat
5% GC    Probe # 36, 35% GC    Probe # 37, 40% GC          Probe # 38
cttcgatttcctctaaacagtttaatgactcgatcacatttgtttcctcaattctatga
gaagctaaag  gatttgtcaaattactgagc  gtgtaaacaaaggagttaag   
, 35% GC    Probe # 39, 35% GC    Probe # 40, 35% GC

Latest revision as of 20:57, 5 March 2010

Progress:

Probes designed: (03-04-2010 - Christoph Engert)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

>T01D3.2 (spliced + UTR - 985) gagaaATGGA GACTAATCTT TCTGAAGAAA AACAAAAACC GTCAAAATCA CAAGCCCAGC AGAGAAGACA AATGGAAAAT TATGAATTCT CACAGCTAGC CAATGAACTT CCATTGGCAA GAGCAATTTC TGGACAGCAT ATTGATAAAA CAACGATGGT TAGATTAGCA ACTGCTTATA TCAAACTTCA CAATATATTT GGGCAATCTC AAAGAGCCTA CAGTTCAGCT GACTATTATT ATGGAAGTGA CTCTTTATGG ACTAACAATC ATTTAGATCT ACTAGATGGA TTCTTTGTGA TACTGGACAG GCGCGGAGAT GTACTTTACA TAAGTGAAAC TATTAGTATT TACTTGGGCT TGTCACAAGT TGAAATGACT GGCAACGCGA TGGTTGATTA TATTCATGAA CAGGATATCA ATTGCTTTAA CTCGGCCCTT AACTATTGTG ACCTTAATTG GCCTCAAATG TGCAACGTAC GTGTTAAATC TAGTCTCACA AAGCGAGCAA ACAAGGATGC AGTTCGTGCT TCACCTGGAT ACAAGGTTCT TCGTCTAGAA ATTACAATGG GTCCAAATAC AAATACTAGA ATGATTGCAT GTTATCCAAT GCCTACTCCA GTTCTTTCTA CAGTAACCAT TCCTTCTAAT TCATTTGTTA TCATTACATC CATCGATCTT CATATAACAT TTGCTGATGA AAAAGCTCAT CAGTTGCTTA ATAACCCATT TTACCCAGAT TCAAATATCA AAGGAATGTC ATTATATTCA CTGATTGATA TCTCAGATTC TGAAGTTATA TCAAAAATGC ATTTCGATAT TTTCAACCTG GGCGCTTATA AAACCCCGTA CTATCGAATG ATCTTGAATC AAACAAGTGA AACTTTTTAC GTCGAGTCAA ATATTTTTCG TCATACTTCG ATTTCCTCTA AACAGTTTAA TGACTCGATC ACATTTGTTT CCTCAATTCT ATGAcctcca acttt

Name of probe set: hlh-34

Probes designed on Wed, 03 Mar 10 23:49:08 -0700

40 probes designed for target of length 969

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

cttcagaaagattagtctcc, hlh-34_1, 1, 3, 40

ggcttgtgattttgacggtt, hlh-34_2, 2, 32, 45

tttccatttgtcttctctgc, hlh-34_3, 3, 54, 40

ggctagctgtgagaattcat, hlh-34_4, 4, 77, 45

ctcttgccaatggaagttca, hlh-34_5, 5, 99, 45

tcaatatgctgtccagaaat, hlh-34_6, 6, 121, 35

gctaatctaaccatcgttgt, hlh-34_7, 7, 145, 40

gtgaagtttgatataagcag, hlh-34_8, 8, 167, 35

ctttgagattgcccaaatat, hlh-34_9, 9, 190, 35

atagtcagctgaactgtagg, hlh-34_10, 10, 212, 45

gtccataaagagtcacttcc, hlh-34_11, 11, 238, 45

ccatctagtagatctaaatg, hlh-34_12, 12, 265, 35

cctgtccagtatcacaaaga, hlh-34_13, 13, 287, 45

ttatgtaaagtacatctccg, hlh-34_14, 14, 309, 35

gcccaagtaaatactaatag, hlh-34_15, 15, 335, 35

gccagtcatttcaacttgtg, hlh-34_16, 16, 359, 45

gaatataatcaaccatcgcg, hlh-34_17, 17, 381, 40

aagcaattgatatcctgttc, hlh-34_18, 18, 403, 35

acaatagttaagggccgagt, hlh-34_19, 19, 425, 45

acatttgaggccaattaagg, hlh-34_20, 20, 447, 40

gactagatttaacacgtacg, hlh-34_21, 21, 471, 40

tccttgtttgctcgctttgt, hlh-34_22, 22, 493, 45

tccaggtgaagcacgaactg, hlh-34_23, 23, 515, 55

tttctagacgaagaaccttg, hlh-34_24, 24, 537, 40

tttgtatttggacccattgt, hlh-34_25, 25, 559, 35

ggataacatgcaatcattct, hlh-34_26, 26, 583, 35

agaaagaactggagtaggca, hlh-34_27, 27, 605, 45

gaattagaaggaatggttac, hlh-34_28, 28, 628, 35

gaagatcgatggatgtaatg, hlh-34_29, 29, 657, 40

gatgagctttttcatcagca, hlh-34_30, 30, 687, 40

gaatctgggtaaaatgggtt, hlh-34_31, 31, 718, 40

gtgaatataatgacattcct, hlh-34_32, 32, 747, 30

cttcagaatctgagatatca, hlh-34_33, 33, 771, 35

ggttgaaaatatcgaaatgc, hlh-34_34, 34, 804, 35

agtacggggttttataagcg, hlh-34_35, 35, 828, 45

gtttgattcaagatcattcg, hlh-34_36, 36, 850, 35

ctcgacgtaaaaagtttcac, hlh-34_37, 37, 872, 40

gaaatcgaagtatgacgaaa, hlh-34_38, 38, 901, 35

cgagtcattaaactgtttag, hlh-34_39, 39, 923, 35

gaattgaggaaacaaatgtg, hlh-34_40, 40, 945, 35

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