Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/03/04"

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#find equal amount of reactant free gold particles
 
#find equal amount of reactant free gold particles
  
 +
==procedure==
 +
*ran uv vis on our gold nanoparticles from alpha and beta to determine the concentration of our nanoparticles
 +
**they were determined to be around 10^12.
 +
*we are making the gamma method protein nanoparticles
 +
**doing 10^-8 M protein concentration
 +
**adding 9.9mls and 100ul of protein
 +
**stir for 30 minutes at room temperature
 +
**spin for 30 minutes at 6500g
  
 
==Notes==
 
==Notes==

Revision as of 11:43, 4 March 2014

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March 4th

To DO

  1. determine concentration of gold particles
  2. find equal amount of reactant free gold particles

procedure

  • ran uv vis on our gold nanoparticles from alpha and beta to determine the concentration of our nanoparticles
    • they were determined to be around 10^12.
  • we are making the gamma method protein nanoparticles
    • doing 10^-8 M protein concentration
    • adding 9.9mls and 100ul of protein
    • stir for 30 minutes at room temperature
    • spin for 30 minutes at 6500g

Notes

The gold nanoparticle concentration that we were using in alpha and beta method were 10^12...The reactant free nps that we are using are 10^11, but we have diluted them tenfold to 10^10.

  • I think that we should have lesser activity in the gamma method as there is less gold nanoparticles per ml