User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/02/18: Difference between revisions
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*one solution was 10^-7M protein in nanoparticles. The other was 10^-8M. | *one solution was 10^-7M protein in nanoparticles. The other was 10^-8M. | ||
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Making HRP nanoparticles | |||
**First I made the buffer solution. The pH was 5. I made it using 0.0003 moles of acetic acid 1M and 0.0006 moles of potassium acetate | |||
***add 300ul of acid and 0.059g of base | |||
**Going to make 10^-6M HRP solution in the buffer | |||
***HRP molecular mass is 44kDa or 44000g/mol | |||
****added 0.0044g of HRP | |||
***we then filled up 100ml volumetric flask | |||
**Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions | |||
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*one solution was 10^-7M protein in nanoparticles. The other was 10^-8 | |||
==Notes== | ==Notes== |
Revision as of 12:59, 18 February 2014
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