Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/02/18"

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(Procedures)
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==Procedures==
 
==Procedures==
 
Other things to do
 
Other things to do
*take aliquot of 10^-8M HRP nanoparticles and put in freezer  
+
*take aliquot of 10^-8M HRP nanoparticles and put in freezer
 +
<br>
 +
<br>
 +
*Protein Gold Nanoparticles
 +
**First I made the buffer solution. The pH was 5. I made it using 0.0003 moles of acetic acid 1M and 0.0006 moles of potassium acetate
 +
**Going to make 10^-6M HRP solution in the buffer\
 +
***we then filled up 100ml volumetric flask
 +
**Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions
 +
<br>
 +
*one solution was 10^-7M protein in nanoparticles. The other was 10^-8M.
  
 
==Notes==
 
==Notes==

Revision as of 11:42, 18 February 2014

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February 18th

Goals

  • Make graph showing max peak over time
  • Making HRP nanoparticles using dopamine method
  • Run UV vis on HRP nanoparticles to see if there is any difference after having it run a week later
  • Run UV vis on HRP supernatant
    • We want to see there is free HRP in the solution and that all of the HRP did not get spun down



  • make hemoglobin nanoparticles using the alpha method

Procedures

Other things to do

  • take aliquot of 10^-8M HRP nanoparticles and put in freezer



  • Protein Gold Nanoparticles
    • First I made the buffer solution. The pH was 5. I made it using 0.0003 moles of acetic acid 1M and 0.0006 moles of potassium acetate
    • Going to make 10^-6M HRP solution in the buffer\
      • we then filled up 100ml volumetric flask
    • Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions


  • one solution was 10^-7M protein in nanoparticles. The other was 10^-8M.

Notes

  • HRP was 10^-8 M added to nanoparticles

Reflections