Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/19"

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(Notes)
(Notes)
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| 50||0.5||9.5
 
| 50||0.5||9.5
 
|}
 
|}
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 +
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Table 2. Preparation of BSA stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock solution'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
|Molecular weight (g/mol)||66000
 +
|-
 +
| mass (g)||0.0432
 +
|-
 +
| volume stock (L)||0.03
 +
|-
 +
| Concentration (M)||2.18182E-05
 +
|}
 +
<br>
 +
Table 3. Preparation of Invertase Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation Invertase Stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular weight (g/mol)||135000
 +
|-
 +
| mass invertase (g)||0.1015
 +
|-
 +
| volume stock (L)||0.025
 +
|-
 +
| concentration (M)||3.00741E-05
 +
|}
 +
<br>
 +
Table 4. Preparation of BSA-AuNP solutions
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''concentration BSA (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume BSA (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 +
|-
 +
| 105||0.347660664||2.38095E-06||0.545634921||4.106704416
 +
|-
 +
| 110||0.347660664||2.27273E-06||0.520833333||4.131506003
 +
|-
 +
| 115||0.347660664||2.17391E-06||0.498188406||4.15415093
 +
|-
 +
| 120||0.347660664||2.08333E-06||0.477430556||4.174908781
 +
|-
 +
| 125||0.347660664||0.000002||0.458333333||4.194006003
 +
|-
 +
| 130||0.347660664||1.92308E-06||0.440705128||4.211634208
 +
|-
 +
| 135||0.347660664||1.85185E-06||0.424382716||4.22795662
 +
|-
 +
| 140||0.347660664||1.78571E-06||0.40922619||4.243113146
 +
|-
 +
| 145||0.347660664||1.72414E-06||0.395114943||4.257224394
 +
|-
 +
| 150||0.347660664||1.66667E-06||0.381944444||4.270394892
 +
|}
 +
<br>
 +
Table 5. Preparation of Invertase-AuNP solutions
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Invertase (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Invertase (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume water added (mL)'''
 +
|-
 +
| 30||0.347660664||8.33333E-06||1.38546798||3.266871356
 +
|-
 +
| 50||0.347660664||0.000005||0.831280788||3.821058548
 +
|-
 +
| 70||0.347660664||3.57143E-06||0.593771992||4.058567345
 +
|-
 +
| 90||0.347660664||2.77778E-06||0.46182266||4.190516676
 +
|-
 +
| 110||0.347660664||2.27273E-06||0.377854904||4.274484432
 +
|-
 +
| 130||0.347660664||1.92308E-06||0.31972338||4.332615956
 +
|-
 +
| 150||0.347660664||1.66667E-06||0.277093596||4.37524574
 +
|-
 +
| 170||0.347660664||1.47059E-06||0.244494349||4.407844987
 +
|-
 +
| 190||0.347660664||1.31579E-06||0.218758102||4.433581234
 +
|-
 +
| 210||0.347660664||1.19048E-06||0.197923997||4.454415339
 +
|}
 +
<br>
 +
 
__NOTOC__
 
__NOTOC__

Revision as of 14:19, 1 December 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Entry title

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Procedure

New Nanoparticle Synthesis: Please choose two from the following sets!

  1. Bovine serum albumin
    1. Ratios
      1. 105
      2. 110
      3. 115
      4. 120
      5. 125
      6. 130
      7. 135
      8. 140
      9. 145
      10. 150
  2. Catalase
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
  3. Invertase
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
  4. Elastin
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210

Notes

Table 1. Preparation of Gold Standards

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5


Table 2. Preparation of BSA stock solution

Preparation of BSA stock solution '
Molecular weight (g/mol) 66000
mass (g) 0.0432
volume stock (L) 0.03
Concentration (M) 2.18182E-05


Table 3. Preparation of Invertase Stock solution

Preparation Invertase Stock '
Molecular weight (g/mol) 135000
mass invertase (g) 0.1015
volume stock (L) 0.025
concentration (M) 3.00741E-05


Table 4. Preparation of BSA-AuNP solutions

Ratio Amount of gold added(mL) concentration BSA (M) volume BSA (mL) volume Water added (mL)
105 0.347660664 2.38095E-06 0.545634921 4.106704416
110 0.347660664 2.27273E-06 0.520833333 4.131506003
115 0.347660664 2.17391E-06 0.498188406 4.15415093
120 0.347660664 2.08333E-06 0.477430556 4.174908781
125 0.347660664 0.000002 0.458333333 4.194006003
130 0.347660664 1.92308E-06 0.440705128 4.211634208
135 0.347660664 1.85185E-06 0.424382716 4.22795662
140 0.347660664 1.78571E-06 0.40922619 4.243113146
145 0.347660664 1.72414E-06 0.395114943 4.257224394
150 0.347660664 1.66667E-06 0.381944444 4.270394892


Table 5. Preparation of Invertase-AuNP solutions

Ratio Amount gold added (mL) Concentration Invertase (M) Volume Invertase (mL) Volume water added (mL)
30 0.347660664 8.33333E-06 1.38546798 3.266871356
50 0.347660664 0.000005 0.831280788 3.821058548
70 0.347660664 3.57143E-06 0.593771992 4.058567345
90 0.347660664 2.77778E-06 0.46182266 4.190516676
110 0.347660664 2.27273E-06 0.377854904 4.274484432
130 0.347660664 1.92308E-06 0.31972338 4.332615956
150 0.347660664 1.66667E-06 0.277093596 4.37524574
170 0.347660664 1.47059E-06 0.244494349 4.407844987
190 0.347660664 1.31579E-06 0.218758102 4.433581234
210 0.347660664 1.19048E-06 0.197923997 4.454415339