User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16

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We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol


  1. Add experimental record here. Include what, how, and why...


Stock Solution

  1. Buffer
    1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
  2. Luminol
    1. Dissolve 12.9mg luminol in 300uL of DMSO
    2. Add to 50mL buffer ---> 1.46mM
  3. H2O2
    1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
    2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM


Figure 1: Absorbance spectra of the catalytic activity of HRP-AuNPs

- We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300

Figure 2: Graph showing the absorbance spectra for citrate AuNP and HRP AuNP

HRP and Citrate AuNP.png

Making the BSA AuNP solution

-Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask

-Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA

   - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA
     [BSA] = 15.6μM
        - (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution

Add deionized water up to 10mL

Transfer solution to a test tube and cap with aluminum foil

Heat in oven at 80C for 3 hours